The influence of composition of poly(n-isopropylacrylamide-co-itaconic acid) hydrogel on immobilized Candida rugosa lipase activity
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چکیده
منابع مشابه
Catalyzed Ester Synthesis Using Candida rugosa Lipase Entrapped by Poly(N-isopropylacrylamide-co-itaconic Acid) Hydrogel
This study reports the synthesis of polymeric matrices based on N-isopropylacrylamide and itaconic acid and its application for immobilization of lipase from Candida rugosa. The lipase was immobilized by entrapment method. Free and immobilized lipase activities, pH and temperature optima, and storage stability were investigated. The optimum temperature for free and entrapped lipase was found to...
متن کاملStimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions
Stimuli responsive pH- and temperature-sensitive hydrogel drug delivery systems, as those based on N-isopropylacrylamide (NiPAAm) and itaconic acid (IA), have been attracting much of the attention of the scientific community nowadays, especially in the field of drug release. By adjusting comonomer composition, the matrix is enabled to protect the incorporated protein in the highly acidic enviro...
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Hydrophobic magnetic silica aerogel was used as a support to immobilize Candida rugosa lipase by adsorption method. Physical and chemical properties of the support and immobilized lipase were determined by Field Emission Scanning Electron Microscope (FESEM), Brunauer–Emmett–Teller (BET) analysis and Fourier Transform InfraRed (FT-IR) spectroscopy and the results showed that the lipase was s...
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Effects of preparative parameters on the properties of chitosan hydrogel beads containing Candida rugosa lipase.
The influences of the pH, tripolyphosphate (TPP) concentration, and ionic strength of the gelling medium on the entrapment efficiency, release, and activity of lipase in chitosan hydrogel beads were studied. A solution of Candida rugosa lipase was prepared in a 1.5% w/v chitosan and 1% (v/v) acetic acid medium, and dropped into a TPP solution. Release of lipase in pH 7.2 Tris buffer was monitor...
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ژورنال
عنوان ژورنال: Hemijska industrija
سال: 2008
ISSN: 0367-598X,2217-7426
DOI: 10.2298/hemind0806339m